應用分類
Sequencing Platform
Benchtop sequencing systems
Key specifications | iSeq 100 System | MiniSeq System | MiSeq System | MiSeq i100 Series | NextSeq 1000 and 2000 Systems |
|---|---|---|---|---|---|
Number of kit configurations | 5 | 5 | 9 | 10 | 14 |
Output range | 144 Mb–1.2 Gb | 1.65–7.5 Gb | 300 Mb–15 Gb | 1.5–30 Gb | 10–540 Gb |
Run time (range) | 9.5–19 hr | 5–24 hr | 5.5–56 hr | ~4–15.5 hr | 8–44 hr |
Max reads per run (single reads) | 4M | 25M | 25M | 100M | 1.8B |
Max read length | 2 × 150 bp | 2 × 150 bp | 2 × 300 bp | 2 × 300 bp | 2 × 300 bp |
Production-scale sequencers
Key specifications | NextSeq 1000 and 2000 Systems | NovaSeq 6000 System | NovaSeq X Series |
|---|---|---|---|
Max read length | 2 × 300 bp | 2 × 250 bp | 2 × 150 bp |
Max reads per run (single reads) | 1.8Ba | 10B (single flow cell)b
20B (dual flow cells)b | 26B (single flow cell)c
52B (dual flow cells)c,f |
Run time (range)d | ~8–44 hr | ~13–44 hr | ~17–48 hr |
Max output per flow cell | 540 Gba | 3 Tbb | 8 Tbc |
Sequencing platforms
Trusted technology and proven performance empower scientists to explore further, with confidence. View benchtop and production-scale sequencers, compare features, and learn how to choose the right next-generation sequencing (NGS) platform for your needs.
Sample Prep
Sage Science products are built on the idea that science is more robust and reliable when it is based on high-precision, automated, reproducible technology instead of manually intensive steps that introduce variability and error.Sage Science look at molecular biology workflows and identify high-value steps that can be significantly improved with accurate, automated solutions. The products not only reduce hands-on time and boost throughput, but they also improve data quality and downstream results.The Sage Science portfolio includes products to perform DNA or cDNA sizing and protein fractionation.
Technology
DNA Size Selection System
Pippin Prep
DNA Size Selection System
BluePippin
Library Prep
Explore next-generation sequencing library prep kits that allow scientists to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Optimized for Illumina sequencing platforms, our library prep kits support a large range of throughput needs and sample types while boosting workflow efficiency.
Library preparation kits
DNA
Application | Whole-genome sequencing | Whole-genome sequencing | DNA enrichment - no UMI |
|---|---|---|---|
TSO 500 HRD -- NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000 SP Flow Cell | NovaSeq 6000Dx in RUO Mode a
NovaSeq X Seriesa | ||
FFPE (DNA and RNA) | FFPE (DNA and RNA) | Blood (cfDNA) | ~2 hrs |
523 genes for DNA variants | 523 genes for DNA variants | 523 genes for DNA variants | ~6.5 hrs |
55 genes for RNA fusions and splice variants | 55 genes for RNA fusions and splice variants | 23 genes for DNA fusions | 10 ng to 1000 ng |
SNVs
InDels
CNVs
Fusions
Splice variants
Genomic signatures
| SNVs
InDels
CNVs
Fusions
Splice variants
| SNVs
InDels
CNVs
Fusions (from DNA)
| Yes |
TMB (Immuno-Oncology)
MSI (Immuno-Oncology)
HRD - Through addition of TSO 500
HRD kit | TMB (Immuno-Oncology)
MSI (Immuno-Oncology) |
TMB (Immuno-Oncology)
MSI (Immuno-Oncology) | |
1.94 Mb DNA; 358 kb RNA | 1.94 Mb DNA; 358 kb RNA | 1.94 Mb DNA | Yes |
4–5 days | 4–5 days | 3-4 days | No |
TSO 500 -- NextSeq 500, NextSeq 550, NextSeq550Dx in Research Mode
TSO 500 HRD -- NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000 SP Flow Cell | NextSeq 1000 & 2000a
NovaSeq 6000
NovaSeq 6000Dx in RUO Mode a
NovaSeq X Seriesa | NovaSeq 6000
NovaSeq 6000Dx in RUO Mode a
NovaSeq X | Yes – on bead |
TSO 500 -- 8 samples/run
TSO 500 HRD -- 8-16 samples/run | 8-36 samples/run (NextSeq 1000 & 2000)
16–192 samples/run (NovaSeq 6000)
32-480 samples/run (NovaSeq X)
32-960 samples/run (NovaSeq X Plus) | 8–48 samples/run | Illumina DNA Prep with Enrichment |
40 ng DNA
40 ng RNA | 40 ng DNA
40–80 ng RNA | 20 ng cfDNA (10–30 ng possible) | |
99.9998% | 99.9998% | 99.9994% (SNVs) | |
>96% | >96% | >95% |
RNA
Application | Whole transcriptome | mRNA | RNA enrichment |
|---|---|---|---|
Hands-on time
| < 3 hrs | < 3 hrs | < 2 hrs |
Turnaround time | ~7 hrs | 6.5 hrs | < 9 hrs |
Input | 1 to 1000 ng standard quality RNA; 10 ng for optimal performance and FFPE samples | 25 to 1000 ng standard quality RNA | 10 ng standard quality RNA; 20 ng RNA for low quality / degraded / FFPE |
Automation capability | Liquid handling robots | Liquid handling robots | Liquid handling robots |
PCR protocol | No | No | Yes |
Library Quant needed? | Yes | Yes | Yes |
Fragmentation included? | Yes | Yes | Not required |
Product | Illumina Stranded Total RNA Prep | Illumina Stranded mRNA Prep | Illumina RNA Prep with Enrichment |
Data Analysis & Interpretation
Customizable Workflows and Integrations
NGS Pipeline Management & Genetic Data Storage
Variant Analysis
Variant Browser
Rapid Phenotype-Driven Interpretation
Advanced ACMG/ClinGen Variant Classification
Comprehensive and Customizable Reports
Long-Read Sequencing Analysis